Journal: Chinese Medicine

Article Title: Salvianolic acid B and Senkyunolide I synergistically alleviate cardiac hypertrophy and regulate MAP3K1 signaling

doi: 10.1186/s13020-025-01189-9

Figure Lengend Snippet: Coadministration of Sal B and Sen I regulates the MAP3K1 signaling. A Heat map showing representative differentially expressed genes in cardiac tissue. B KEGG pathway analysis of RNA-seq data. C Relative levels of MAP3K1, MAPK11 and Max mRNA in mice heart tissue (n = 3 per group). D , E Representative western blot and quantification analyses of cardiac MAP3K1 ( D ) and MAX ( E ) expression (n = 5 per group). The predicted additive effects for Sal B and Sen I (E sal + E sen ) is represented by a black dashed line. Sal B, 80 mg/kg/day; Sen I, 50 mg/kg/day; captopril, 20 mg/kg/day. Statistical significance was analyzed using Student’s t-test in ( D, right and E , right) and one-way ANOVA with Tukey’s post hoc test; # P < 0.05, ## P < 0.01 when compared with the control group; *P < 0.05, **P < 0.01

Article Snippet: MAP3K1 Polyclonal antibody (19970-1-AP) and MAX Polyclonal antibody (10426-1-AP) were purchased from Proteintech of United States.

Techniques: RNA Sequencing, Western Blot, Expressing, Control

Journal: Chinese Medicine

Article Title: Salvianolic acid B and Senkyunolide I synergistically alleviate cardiac hypertrophy and regulate MAP3K1 signaling

doi: 10.1186/s13020-025-01189-9

Figure Lengend Snippet: Synergistic effects of Sal B and Sen I in PE-induced hypertrophic NRCMs. A Representative fluorescence images and corresponding quantification of phalloidin (F-actin) immunostaining in NRCMs. B and C Relative levels of ANF and BNP mRNA. D , E Representative and quantification of the western blot study of MAP3K1 F The efficiency of MAP3K1 knockdown in NRCMs. G Representative fluorescence images and corresponding quantification of phalloidin (F-actin) immunostaining in NRCMs. ( D ) (n = 5 per group) and MAX ( E ) (n = 6 per group). PE, 100 μM; Sal B, 25 μM; Ros acid, 25 μM. Scale bar, 50 μm. Quantitative data are presented as the mean ± SEM. The predicted additive effects for Sal B and Sen I (E sal + E sen ) are represented by a black dashed line. Statistical significance was analyzed using Student’s t-test in CI analysis and one-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01. Scale bar, 100 μm

Article Snippet: MAP3K1 Polyclonal antibody (19970-1-AP) and MAX Polyclonal antibody (10426-1-AP) were purchased from Proteintech of United States.

Techniques: Fluorescence, Immunostaining, Western Blot, Knockdown

Journal: Chinese Medicine

Article Title: Salvianolic acid B and Senkyunolide I synergistically alleviate cardiac hypertrophy and regulate MAP3K1 signaling

doi: 10.1186/s13020-025-01189-9

Figure Lengend Snippet: The molecular docking and dynamics simulation results for Sal B and Sen I towards MAP3K1. A , C and E The representative 3D docking modes of MAP3K1 with Sal B ( A ), Sen I ( C ) and both two compounds ( E ), respectively. The green sticks refer to active site residues and dotted yellow lines indicate hydrogen-bonding interactions between protein and ligand. B , D and F The representative 2D protein–ligand interactions for MAP3K with Sal B ( B ), Sen I ( D ) and both two compounds ( F ), respectively. The colored plates refer to active site residues and dashed lines indicate different interaction bonds. G , H RMSD ( G ) and RMSF ( F ) values of each protein–ligand complex through 100 ns MD simulation

Article Snippet: MAP3K1 Polyclonal antibody (19970-1-AP) and MAX Polyclonal antibody (10426-1-AP) were purchased from Proteintech of United States.

Techniques: